In vitro assessment for cytotoxicity screening of new antimalarial candidates

In antimalarial research there are no standard procedures to determine the toxicity of a drug candidate. Among alternatives available, in vitro cytotoxicity assays most widely used predict toxic effects future therapeutic products. They have advantage over vivo assays, that they offer possibility restrain number experimental variables. The objective present study was compare cytotoxic methods by testing various compounds currently treat malaria against different cell lines. Neutral red (NR) uptake and methylthiazoletetrazolium (MTT) colorimetric were preliminary commercially available drugs tumor non-tumor cells Toxicity through brine shrimp lethality bioassay hemolytic activity also evaluated. Significant differences observed tests measured NR uptake. lines TOV-21G HepG2 WI-26VA4 showed relatively uniform results, with being sensitive tested, presenting lowest concentration cause death 50% viable (CC50) values. results this support use TOV-21G, as choice for evaluate potential bioactive compounds.